1cuatro 10 % (v/v) DMSO (Carl Roth) offered just like the manage plus the stability away from unattended cells are outlined since the 100 %.
Statistical analysis: Data of the bar charts are shown as mean+SD. Potency (ICfifty) data are presented as mean with the confidence interval (95 %). Statistical analysis was done by using GraphPad Prism 6.0 (GraphPad software, San Diego, USA). Nonlinear regression was used to plot and analyze concentration-response curves and to obtain IC50 values.
Computational methods: The crystal structure of the heterodimer of TLR2-TLR1 with bound Pamstep threeCSK4 (PDB ID: 2Z7X) 15 was retrieved from the Protein Data Bank 16 and used for docking studies with the tested compounds. Prior to docking the TLR1 monomer, all ligands and water molecules were removed using Molecular Operating Environment (MOE2019, Chemical Computing Group, Montreal, QC, Canada). The TLR2 monomer was protonated using the “Protonate 3D” application included in MOE2019. Continue Reading
